Operating a bioreactor is an intricate balancing act. Every parameter must be meticulously controlled to guarantee a successful outcome. One of the most critical challenges in bioprocessing is contamination control. A single microscopic invader can cause serious problems in your bioreactor, potentially ruining weeks or months of work. Here are five ways to prevent contamination in your bioreactors.
Sterilization is the frontline defense against contamination. Bioreactors and all related equipment should be sterilized before use, either through autoclaving, chemical sterilants, or gamma irradiation. Additionally, the media used should also be sterilized to ensure that no potential contaminants are introduced.
Human error is often the source of contamination. Regular training of all personnel in good laboratory practices that emphasizes the importance of personal hygiene, correct handling of materials, and proper use of equipment can significantly reduce the risk of contamination.
The materials and media used in bioreactors are often nutrient-rich, making them a potential breeding ground for contaminants. Therefore, proper handling and storage are vital. For instance, media should be pre-sterilized, stored in a sterile environment, and added to the bioreactor using aseptic techniques.
Regular maintenance and monitoring of the bioreactor can help in early detection of contamination. This includes routine microbial testing of the bioreactor contents and examining the physical state of the bioreactor for any leaks or damages that might allow contaminants to enter.
Jacketed bioreactors provide an extra layer of control over the process conditions. The jacket allows for precise temperature regulation, which is essential for preventing the growth of thermophilic contaminants. Additionally, by maintaining the optimal temperature for your bioprocess, you discourage the growth of organisms that thrive in other conditions.
Now that you know these five ways to prevent contamination in your bioreactors, work with International Filter Products to get the jacketed bioreactors you need—we have them in stock now. If you’d like to learn more about any of our products, you can always give us a call at (818) 504-8115.